IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Gold Bioremediation/2010/09/26

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9.26.10

A miniprep verification PCR was conducted for golS + Terminator + pSBIT3, golB + Terminator + pSBIT3, pgolS + E0420 + pSBIT3, pgolS + E0240 + pSBIT3, and pgolS + E0430 + pSBIT3.

GesA, gesB, and gesC PCRs were also purified.

Minipreps of the golS + LamB and the golB + LamB was eluted with 30 uL of H2O.

A second PCR minprep verification was done for for golS + Terminator + pSBIT3, golB + Terminator + pSBIT3, pgolS + E0420 + pSBIT3, pgolS + E0240 + pSBIT3, and pgolS + E0430 + pSBIT3.