IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Gold Bioremediation/2010/09/23

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9.23.10

A miniprep of pgolS + E0430 + pSBIT3 was completed eluting with 20 uL of H2O and an overall 35 ng/uL of the desired sequence was yielded. Digestions to verify pgolS + E0430 insert was also conducted using 20 uL reactions.

A electrophoresis gel of gesA, gesB, and gesC (from 9/22/10 PCR) and of golS with BSA for LamB was carried out.

A miniprep of golT was also sequenced using golT miniprep from 8/12/10.

Lastly a gel was ran for the pgolS + E0430 miniprep.