IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Gold Bioremediation/2010/09/22
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PCR of gesA, gesB, gesC, and golS with BsaI site was done using 50 uL reactions.
Gel electrophoresis was done for golT verification that included samples from 8/12 and 8/17 and golB with BsaI sites verification. A golS miniprep was used as a positive control for the experiment and also a control with no DMSO was used. .