IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Gold Bioremediation/2010/09/10

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9.10.10

Gel electrophoresis of the PCRs from 9/9/10 was done. This was done as a way to analyze our results from 9/9/10 PCR to see if we had any amplification. This amplification would help determine if an insert was present in the plasmid or not.

PCR purification was also done of the PCRs from 9/9/10. The purification of the PCR helps make the samples purer of any undesired nucleotide sequences in the PCR mixture.

The purifications were then used for sequencing of the nucleotides in golS, golB, and pgolS. Sequencing allowed for determination of the nucleotide sequence of the samples and allowed for comparison with the actual known sequences. There was satisfactory matching found for all three of the genes that were sequenced.