IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/09/12

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9/12/10

1:35

Transformation/Plating

50 μL cells, 3 μL ligation. 10 min ice, 45 sec 42°C, 2 min ice. add 1 mL LB, incubate at 37°C shaking for 1 hour.

plated 250 μL