IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/09/11

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9/11/10

10:15 AM

Digestion B0034ArsRSDM2B0034pSB1C3

DNA 1.8 μL
H2O 39.7 μL
Buffer 4 5 μL
BSA .5 μL
XbaI 1 μL
PstI 2 μL

J23100

DNA 2.6 μL
H2O 39.9 μL
Buffer 4 5 μL
BSA .5 μL
EcoRI 1 μL
SpeI 1 μL

pSB1T3

DNA 1 μL
H2O 40.5 μL
Buffer 4 5 μL
BSA .5 μL
EcoRI 1 μL
PstI 2 μL

incubate at 37°C for 15 min, inactivate at 80°C for 20 min

11:10 AM

Ligation

B0034ArsRSDM2B0015 2 μL
J23100 2 μL
pSB1T3 2 μL
Ligase Buffer 2 μL
Ligase 1 μL
H2O 11 μL

Incubate at room temp for 15 min, inactivate at 80°C for 20 min