IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/09/03

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9/3/10

8:55 AM

Growth on ArsR-B0015-pSB1A2 plate. good streaks on all plates except B0015 on Amp (no growth)--B0015 grew on Amp but not Kan even though the resistance should be Amp/Kan...

10:00 AM

Gel

Lane Sample Amount
1 1 Kb Ladder 1 μL
2 ArsB 1 4 μL dye, 20 μL sample
3 ArsB 2 4 μL dye, 20 μL sample
4 ArsB 3 4 μL dye, 20 μL sample
5 ArsB 4 4 μL dye, 20 μL sample
6 Negative Control 4 μL dye, 20 μL sample

3:00 PM

Made 5 mL cultures (3X of ArsR-B0015)

Re-streaked B0015, B0034 and gas vesicles.