IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/08/28

From OpenWetWare
Jump to: navigation, search
UIUC ArsR System.jpg UIUC - Arsenic Collection System! Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

8.28.10

gel

Lane Sample Amount
1 1 Kb Ladder 1 μL
2 ArsB1 5 μL sample + 1 μL dye
3 ArsB2 5 μL sample + 1 μL dye
4 ArsB3 5 μL sample + 1 μL dye
5 ArsB4 5 μL sample + 1 μL dye
6 Negative control 5 μL sample + 1 μL dye

UIUC 8.28.10 arsb.jpg

Overnight of ArsRB0015 was red, will try using a different backbone next time

11:30 AM

made three 5 mL cultures of ArsRSDM2, grew overnight at 30°C

Streaked MG1655 on LB from glycerol stock to use in genome prep later next week.