IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/08/21

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8/21/10

10:15 PM

Got good growth on the control plate. Also observed colonies on 1 plate each for pArsRF1R2 and pRpoS438 site directed mutagenesis and LamB/ArsR fusion plates.

ArsR-B0015-pSB1T3 cultures all red, must have picked red colonies accidentally.

2:35 PM

Started 5 mL cultures:

pRpoS 2 colonies

pArsR 2 colonies

ArsR/LamB fusion 2 colonies

ArsR-B0015 4 colonies.