IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/08/03
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Incubate at 37°C for 15 min, inactivate at 80°C for 20 min.
pRpoS438 colony 1: 214.4 ng/μL
pRpoS438 colony 2: 380.3 ng/μL
pArsRF1R2 colony 1: 246.9 ng/μL
pArsRF1R2 colony 2: 456.2 ng/μL
Incubate at room temperature for 10 min, inactivate at 80°C for 20 min.
Transformation/Plating 100 μL cells, 3 μL Ligation in cuvette. electroporate and add 1 mL SOC. Recover at 37°C for 1 hour.
Plated 100 μL on 1 half of a cap plate, 50 μL on the other half of the plate at 1:10 PM
Verification of insert
Per Reaction: 19 μL mastermix, 1 μL template.
pRpoS438 colony 1
pRpoS438 colony 2
pArsRF1R2 colony 1
pArsRF1R2 colony 2
negative control (no template)
started new 5 mL cultures of pArsRF1R2 and LamBF1R for verification PCR tomorrow. Will re-run today's samples with new pArsR and LamBF1R samples as well as old.