IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/06/25

UIUC - Arsenic Collection System!

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6/25/10 12:40 PM inoculated 5 mL broth with colonies from yesterday's biobrick plates. 37°C room to incubate PCR PCR of Plasmid Backbone Component 20ul Rxn 6x Master Mix 10x Buffer 2ul 12ul 10mM dNTP --- --- F Primer (52358135) 1ul (10uM) 6ul R Primer (52358136) 1ul (10uM) 6ul Template DNA (Plamsid Backbone) .5ul (~~250ng) (3) Pfu Turbo DNA Pol 0.2ul 1.2ul dH2O 15.3ul 91.8ul PCR Program Used 95 degC 5 min 95 degC 30 sec 32 cycles 55 degC 30 sec 32 cycles 72 degC 270 sec 32 cycles 72 degC 5 min 4 degC hold Miniprep of Parts for Backbones Minipre of BB Plasmids for Backbone Biobrick Part Backbone Concentration in ng/ul Label RFP pSB1A2 430 A RFP pSB1C3 513 C RFP pSB1T3 428 T E0034 pSB2K3 268 K Gel Lane Sample Amount 1. 1 Kb Ladder 3 ul 2. A 10 ul sample, 2 ul dye 3. C 10 ul sample, 2 ul dye 4. T 10 ul sample, 2 ul dye 5. K 10 ul sample, 2 ul dye 6. A' 10 ul sample, 2 ul dye 7. C' 10 ul sample, 2 ul dye 8. T' 10 ul sample, 2 ul dye 9. K' 10 ul sample, 2 ul dye 10. negative control 10 ul sample, 2 ul dye Ran at 175V 10:10 PM Moved inoculations from this afternoon (12:40) from 37°C room to 4°C room.