IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/06/16
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Promoter BBa_J23119: had growth on both plates for new and used cuvettes. Took 1 colony from new cuvette plate (50 ul plated) and inoculated 5 mL LB broth.
Gas Vesicles BBa_I750016:
made glycerol stock: .9 mL 10% glycerol and .9 mL culture in tube in -80°C freezer.
Miniprepped (promega kit) remaining sample and nanodropped (432.57 ng/ul)
used 100ng DNA as template
19.5 ul mastermix and .5 ul template DNA to each reaction (except negative control which gets .5 ul H2O instead).
Samples: BBa_I750016, negative control.
Primers: Biobrick sequencing forward and reverse.