IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/06/14

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7:00 PM

Pulling parts out of registry

Gas Vesicles: BBa_1750016, Well 11A on Amp plasmid BBa_J61035

10 ul H20 into well 11A.

transformed 2 ul plasmid from kit plate into 100 ul competent cells via electroporation. Added 1 mL LB for recovery and incubated for 1 hour at 37°C. Plated 200 ul and 50 ul onto Amp plates.