IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/05/28
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Bootcamp Day 5
Set up plate for plate reader
Our samples were in lanes 2 and 3, rows B-G, 250 ul of sample in each well.
Miniprep. 22 ul final volume, used 2 ul to nanodrop.
12M 324 ng/ul 12O 353 ng/ul
added 27.8 ul mastermix, .2 ul polymerase, and 2 ul DNA template to each reaction.
Samples: 12M, 12O
Primers: Biobrick Prefix, Biobrick Suffix