IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/07/22

From OpenWetWare
Jump to: navigation, search

Week 6 Day 2

- Transformation of new DNA from DNA 2.0 into E.coli. Tubes 1 to 7.

1. AtPR1

2. ArvBS3

3. NbBi1-RNA

4. NbProB-RN

5. AvrXa27 T

6. OsXa27 Pro

7. Control (No DNA)

- Followed heat shock protocol:

- 5 µl DNA added to 50 µl of chemically competent E.coli

- Spreadplated onto agar plates containing KAN

- Incubated O/N at 37°C

Human Practices

Met with Dr Kay Yeoman, BIO outreach officer to discuss activities for school outreach activities

Discussed activities for 'Food for Thought' event at the cut on 26/07