IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/07/04

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Week 3, Day 5

TSL


-Digest of PCR samples from 3.7.14

-Gel purification and quantification of digested fragments (now ready to ligate into PSB1C3 backbone)

-Digest of PSB1C3 plasmid with EcoR1 and PST1 (removes blunt ends)

-Dephosphorylation of plasmid backbone


-Colony PCR of colonies picked from agro plates (transformed 10.7.14)to confirm that the DNA is what we want.