IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/07/03

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Week 3, Day 4

TSL


- Set up a 20 µl PCR reaction using primers synthesized from Sigma and a level 1 acceptor as the template DNA

• Pro + 5UTR

• CDS

• Ter + 3UTR

-Ran PCR product on a gel to check amplification

-PCR product purification using phenol chloroform