IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/06/27

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Week 2 Day 4


TSL


- Found poor blue/white selection on dig-ligs from 26.06.14. Suspected to be due to either the enzyme used or PCR block. - Set up a new set of dig-ligs to test the source of this problem.

 • Li - block L, iGEM enzyme
 • Lmy - block L, MY enzyme 
 • Bi - block B, iGEM enzyme 
 • Bmy - block B, MY enzyme

- Transformed into E.coli using electroporation

- Spread plate onto plates prepared with xGAL, IPTG