IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/06/26

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Week 2 Day 4


- Checked Dig-Lig tranformation plates grown O/N and poor blue white selection observed with many blue colonies.

- Selected 2 white colonies from each plated and colony PCR performed.

- Following colony PCR, amplified fragments ran on gel to determine size and therefore whether they contained the construct.

- Decided to repeat the 11 Dig-Lig experiments

- Ran digested PCR product from 25.06.2014 on a diagnostic 1% agarose gel in TBE buffer and determined that LacZ had internal restriction enzyme cut sites and product was split into two bands.