IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/06/24

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Week 2 Day 2

TSL


- Selected a single colony from each of the eleven plates and the colonies were grown in TY broth with Kanamycin O/N

- Transformation of pSB1C3 plasmid containing RFP into E.coli using electroporation. Incubated with 50 μl of Soc broth at 37 °C for 1 hour.

- Poured 11 agar plates containing chloramphenicol

- Spreadplated E.coli cells which have been transformed with the pSB1C3 + RFP plasmid

- Completed calculations for Dig-Lig experiments (Size and conc. of DNA parts calculated,size of acceptor calculated, order of constructs for each reaction determined, dilution and DNA conc. for each reaction determined) Ratio of 2:1, constructs:acceptor (plasmid)