IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/27
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The Bioassay plates had to be overlayed with Candida albicans. Made 3 x 200 ml SNA (soft nutrient agar)- 200 ml distilled water, 1 g agar and 1.6 g broth powder. Autoclaved for 1 hr and then left to cool in 55 °C water bath (being careful not to let it become molten, as this kills Candida). A 200 ul volume of the Candida culture and 5 ml of SNA were added per plate and dispensed by gentle swirling. The Bioassays were then incubated at 30°C.