IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/20
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Made 6 x 500 ml SFM agar media, autoclaved for 1 hr and poured into plates.
Isolated more single colonies that resembled Streptomyces from soil sample plates.
We inoculated cultures of BL21 containing pETAntA and Antgp+RFP into 10mls LB with antibiotics Kanamycin and Chlorophenicol. Four cultures were grown overnight at 37°C.