IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/06

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Floor One

Made 1 % agarose gel - 2 x large - 2 x 100 ml TBE buffer and 1 g agarose. Melted in microwave til agarose dissolved.

Prepared spore stocks of all lawns.

Did as much lawning and streak purifying as was possile with available SFM + Ny media.

Made 6 x 500 ml SFM agar, autoclaved for 1 hr and poured into plates.

Loaded and ran gels - added 4 ul loading dye to each sample (46). Used 2 x ladder (1 Kb).

Made up more liquid Nystatin (50 mg/ml) - 10 ml DMSO and 500 mg Nystatin.