IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/06/24
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Transformation reactions were performed on all the specific ligation reactions from the day before, all controls (positive & negative) in addition to the J04450 plasmid only. They were put into incubation overnight at 37 °C.
Also, samples were run on an agarose gel to confirm ligation reactions, but unfortunately the gel image did not give us any information.