IGEM:University of Chicago/2009/Notebook/Paraoxon Biosensor/2009/08/11

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August 11th, 2009 Insert GFP Superfolder PCR (Add on PacI) Gel Image Nora ran a gel to confirm if PCR worked for both my genomic tests and for my GFP PCR from colonies. Genomic tests yielded an empty gel, so either no integration, misintegration, or technical error. Perform PCR to check Restriction Digest of GFP for Ligation into Longtine Plasmid 20 ul GFP GFP DNA = 10 uL Buffer 4 = 2 uL BSA (10X) = 2 uL ddH2O = 5 uL Enzyme: PacI = 0.5 uL "" : AscI = 0.5 uL Control GFP-Kan Vector = 1 uL Buffer 4 = 2 uL BSA 10X = 2 uL ddH2O = 14 uL PacI = 0.5 uL Asc I = 0.5 uL Digest at 37 degrees celsius Started at 12:35 p.m.