IGEM:University of Chicago/2009/Notebook/Paraoxon Biosensor/2009/07/09

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- Helped Damon/Annie

- Ordered Primers for PNP Sensors

- plates from 7/7 are empty

- will try incubation O/N after heat shock (Chichi's advice)

- Salmon sperm. DNA looked defect yesterday. Damon makes new batch.

Maybe Kan casette is defect. Try PCR (beads) for His-tag/marker.

Each tube (50 ul)

- 2 beads

- 0.65 ul template (pFA6a-GFP-His from Satoe)

- 5 ul of each primer

- 37.35 ul dd H2O

- Program "iGem 6". Will run on gel.

- also will try transformations again w/Kan marker plate tmrw. New Kan plates

- Need to order Forward primers for double checking biosensor once yeast grows. Check if correct DNA was incorporated.

End of Day