IGEM:University of Chicago/2009/Notebook/Paraoxon Biosensor/2009/07/02

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EcoRI/SalI: NE Buffer EcoRI (or 3) 37°C
  • 049 and controls run in 1/2 vol (25μL)
  • 049-2.5 got 5μL extra water
  • 6min elongation of 58' anneal 30 cycle

Ran a gel:

2009-07-02 19.jpg

-Tony Luo