IGEM:University of Chicago/2009/Notebook/PNP Biosensor/2009/08/03

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August, 3rd, 2009

OD660 Time 12:20 2:20 4:20 6:20 7:50 10:10 11:20
YGR035 Cont 0.186 0.222 .283 .242 0.436 0.882 1.213
YGR035 0.5 mM 0.183* 0.158 .137 .202 0.185 0.190 0.382
YGR346 Cont 0.171 0.301 .412 .974 1.252 1.383 1.478
YGR346 0.5mM 0.204* 0.289 .187 .223 0.277 0.270 0.444
1 Cont 0.366 0.416 .492 .975 1.331 1.452 1.427
1 0.5 mM 0.166* 0.408 .388 .618 1.029 1.218 1.346
2 Cont 0.168 0.337 .283 .324 0.490 0.898 1.223
2 0.5mM 0.189* 0.270 .396 .462 0.746 1.102 1.245
3 Cont 0.237 0.256 .247 .448 0.481 0.887 1.183
3 0.05 mM 0.274* 0.396 .330 .467 0.590 1.102 1.314
3 0.1 mM 0.126* 0.225 .263 .276 0.482 1.008 1.274
3 0.25 mM 0.239* 0.251 .232 .373 0.647 (0.697?) 1.140 1.305
3 0.5 mM 0.232* 0.349 .160 .281 0.408 0.720 1.053
4 Cont 0.229 0.265 .319 .362 0.371 0.586 0.959
4 0.5 mM 0.293* 0.236 .226 .281 0.319 0.236 0.277

Added to 4.5 ml cultures

             0.5 mM = 22 uL
             0.1 mM = 4.1 uL
             0.05 mM = 2.2 uL
             0.25 mM = 11 uL
  • = time of induction

Visual Test for Glow 1.(0.5) Inconclusive: no green glows

2.(0.5) ""

3. (0.1) ""

3. (0.25) ""

3. (0.5) ""

4. (0.5) ""

035 (0.5) ""

346 (0.5) ""

Glowing tests did not confirm any glowing yeast cultures.

End of the Day