IGEM:University of Chicago/2009/Notebook/PNP Biosensor/2009/07/24

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July 24th, 2009

Restreaked a Colony #4 of Transformation 2

One colony stood in Transofmration 2, and it was on plate four. I picked it and streaked it. this streak yielded a lot of growth. I picked three colonies from this and restreaked these. Also, plated transformation, which I mentioned on the last page

Gel Analysis of Nora's PCR - 1.2% Agarose

L 1 2 3 4 5 6 7 L 8 9 10 11 12 13 14 15 16 17

See Nora's notebook for key

Make PEG Mixture Successful! Finished on the 29th filtration

Gel Analysis of Nora's PCR Didn't work because of lack of Sybr Safe. Tried to use E-Z vision, visualized but it didn't really do anything for us.

End of day