IGEM:University of Chicago/2009/Notebook/PNP Biosensor/2009/07/24

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July 24th, 2009 Restreaked a Colony #4 of Transformation 2 One colony stood in Transofmration 2, and it was on plate four. I picked it and streaked it. this streak yielded a lot of growth. I picked three colonies from this and restreaked these. Also, plated transformation, which I mentioned on the last page Gel Analysis of Nora's PCR - 1.2% Agarose L 1 2 3 4 5 6 7 L 8 9 10 11 12 13 14 15 16 17 See Nora's notebook for key Make PEG Mixture Successful! Finished on the 29th filtration Gel Analysis of Nora's PCR Didn't work because of lack of Sybr Safe. Tried to use E-Z vision, visualized but it didn't really do anything for us. End of day