IGEM:University of Chicago/2009/Notebook/Organophosphate Hydrolase/2009/07/13

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July 13, Monday, 2009

Going to look at pCTCON2-GFP by fluorescence microscopy.

Protocol

1. Grow pCTCON2, pCTCON2-GFP transformants to sat'd overnight in sterile-fitted 2x -W / 0.1% glu (so, actual conc. .2%)

2. In the morning, dilute at OD660 to 0.2 - 0.3 in 2x -W/2% gal

3. ~ 3 hours @ 30 degrees C

4. Scope!

Restreaked transformants from Thursday, 9 July 2009 b/c plates too dense and pCTCON2 plate accidentally gouged w/ lid. Control plate had mold. Why so much mold?

End of the Day