IGEM:University of Chicago/2009/Notebook/Organophosphate Hydrolase

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<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext">07/02/2009,07/07/2009,07/08/2009,07/09/2009,07/10/2009,07/12/2009,07/13/2009,07/16/2009,10/05/2009,10/06/2009</div><div style="display:none;" id="page">IGEM:University of Chicago/2009/Notebook/Organophosphate Hydrolase</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

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Description/Abstract

Organophosphate hydrolase

Surface Display

Aga2.jpg

  • For the first step in paraoxon degradation, we will express organophosphate hydrolase on the yeast cell-wall surface using the Aga2 cell-surface display system. Originally designed for antibody display, this system displays the protein of interest flanked on the N-terminus by Aga2, the disulfide of which binds the myristylated surface membrane protein Aga1. Aga1 and Aga2 are cell-surface glycoproteins which facilitate mating type-specific agglutination of Saccharomyces cerevisiae a and alpha cells. Proper surface expression is dependent on Aga1 expression--Aga2 tagged proteins would otherwise be completed secreted outside the cell wall.

Final module

OPHdisplay.jpg

Researchers

  • Damon Wang
  • Annie Kang

Notes

  • Team meeting today at 3:30 (July 31)
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