IGEM:UW/2008/Notebook/iGEM UW Team/2008/06/10

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1. Plasmid isolation from previous day colonies.

2. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polimerease translation fusion with HindIII and SalI

3. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polimerease translation fusion with HindIII and EcoRI

4. Control digest of pMPM-T5 + T7 RNA-polimerase with EcoRI

5. Control digest of pMPM-T5 + T7 RNA-polimerase with BamHI and XhoI

6. DNA electrophoresis of digestion products

7. Choose proper colonies of transformants