IGEM:UW/2008/Notebook/iGEM UW Team/2008/06/02

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1. Transformation of E.coli TOP10 with pZC320

2. LB + ampicillin(30μg/mL) + X-gal + IPTG

3. PCR for AID transcription fusion with translation fusion AID+T7 RNA-polimerase

Primers: AIDlNrH and T7pXbSal

Template DNA: translation fusion AID+T7 RNA-polimerase (pMPM-T5)

Annealing temperature: 55°C

Time: 4 minutes

20 cycles