IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/27

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1. Isolation of hypothetical pMPM-T5 with trancription fusion

2. Isolation of hypothetical pMPM-T5 with translation fusion

3. Control digest of pMPM-T5 with trancription fusion with EcoRI and XhoI

4. Control digest of pMPM-T5 with translation fusion with BamHI

5. Control digest of pMPM-T5 with translation fusion with EcoRI and XbaI

6. Choice of proper clones and preparation for sequencing