IGEM:UNAM LCG/2009/Notebook/Hydrobium etli/2011/09/21
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pBBR1MCS PCR extraction verification
I ran a gel to see if the PCR amplifications of the pBBRMCS backbone (that I did yesterday) were well. Unfortunately nothing appeard in the gel.
I ran the PCR results to see if the backbone was amplified. I made 4 PCRs from 4 different samples exxtracted by Vladimir: pBBR1MCS 1, pBBR1MCS 2, pBBR1MCS DH5α, pBBR1MCS S17.
1. Ladder 500bp 2,3. not interesting for this study 4. pBBR1MCS 1 5. pBBR1MCS 2 6. pBBR1MCS DH5α 7. pBBR1MCS S17 8. control pBBR1MCS S17 (without PCR amplification).
There are no marks, which means that the PCRs did not run :( No idea why.