IGEM:UNAM LCG/2009/Notebook/Hydrobium etli/2011/09/19
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pBBR1MCS-5 plasmid extraction
Vladimir helped me by making a plasmid pBBR1MCS-5 extraction from 4 different colonies: colony 1, colony 2, colony DH5α, colony S17. I ran a gel to see if the extraction were OK. I conclude that the extraction was succesfully done, the plasmid from S17 was the most concentrated.
I ran the gel with this characteristics:
1. ladder 500bp 2. not interesting for this study 3. not interesting for this study 4. pBBR1MCS-5 1 5. pBBR1MCS-5 2 6. pBBR1MCS-5 DH5α 7. pBBR1MCS-5 S17 8. empty
All the marks are visible and in the correct place (between 4000 and 5000 bp) so I conclude that the extractions were good. The plasmid that is more concentrated is the one extracted from S17 (7th lane). The marks that are visible a little bit up the main marks are isoforms (concatenations for example).
The tubes are in my rack, the next step is to make a PCR from this parts to extract the plasmid backbone.