IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/04/19

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Wet lab meeting

  • Our goals for the following weeks are:

1. Do primers for the blue promoter.
2. Do LB plates with kanamycin and ampicilin.
3. Obtain luciferase by PCR or band purification.

1. Extract regulatory region of YcgF (blue promoter) by PCR.
2. Extract reporter plasmids by PCR.
3. Transform reporter plasmids.

1. Extract double terminator [BBa_B0015] by plasmid extraction.
2. Do a restriction assay for the plasmids.

1. Transform luciferase into E. coli strains.
2. Extract plasmids.