IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/04/19

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Wet lab meeting


  • Our goals for the following weeks are:


-Mariana:
1. Do primers for the blue promoter.
2. Do LB plates with kanamycin and ampicilin.
3. Obtain luciferase by PCR or band purification.

-Jorge:
1. Extract regulatory region of YcgF (blue promoter) by PCR.
2. Extract reporter plasmids by PCR.
3. Transform reporter plasmids.

-Augusto
1. Extract double terminator [BBa_B0015] by plasmid extraction.
2. Do a restriction assay for the plasmids.

-Zepeda
1. Transform luciferase into E. coli strains.
2. Extract plasmids.