IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/03/23
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March 23rd, 2010
One way to achive red light is by Luciferin-Luciferase, for this we need to have an LRE (Luciferin regenerating Enzyme). Basic concept of luciferin regenerated
Getting complete the job
All the elements for systems Lux, will be obtained from V. Fischery.
Now, we are working to get the sequences for all bio-parts and deciding if these parts will be synthesized or getting by PCR.
For LovTAP, there is an analytical Thesis to know how to work with LovTAP, our conclusion are:
We can use that system with its respective properties.
We will learn to study a system which is not 100% effective.
Know if system LRE-Luciferase can work in E. Coli and how to couple to the system.
Search for sequences and details of these.