IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/03/16

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March 16th 2010

YFP (in vitro)

YFP lumisnescent system


  • We have a trouble with LovTAP system. Our system to repress and repressor is not 100% effective, because always is an expression independent of light reception.


  • Efficiency of Quantum Yield could be possible in our system.
  • There is an optional green light receptor and transcriptional response by same system.

We need to know

  • Is there one way to work with LovTAP system?
  • Best CDE genes of operon Lux.
  • Luciferin-luciferase. How to be produced in E. Coli?
  • How to anchor XFP on a Luciferase?
  • What are the details of green light receptor?