IGEM:UNAM/2009/Notebook/Modeling logbook Claudia/2010/03/12

From OpenWetWare
Jump to: navigation, search
TeamLogo.jpg UNAM-Genomics-Mexico team Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Objective: Ask for information about LovTap

I have been in touch with Dr. Devin Strickland the designer of LovTAP, in order to get advices to work with LovTap.

He recommended us the following:

You should take a look at Figure 2.4.2 of my dissertation [1] and try to figure out why that experiment didn't work. You may understand it already, but my interpretation is that LovTAP was being expressed at too high a level. I'd suggest getting the expression level down as much as possible. Also, whatever your reporter is (LacZ, GFP, etc.), I'd recommend putting a degradation tag on it, as in the classic Elowitz and Leibler "Repressilator" system.

See below the figure 2.4.2 information that Devin cited.

Figure2.4.2.Gene repression in vivo by LOV2-TrpR fusion constructs. Transcription of lacZ from a trp promoter was monitored by β-galactosidase activity. Cultures were grown either in the dark or under a broad-spectrum fluorescent light, as indicated. Constructs are identified by the first included TrpR residue at the point of fusion[1].

References

1.STRICKLAND, DEVIN. 2009. Doctoral Dissertation: NEW APPROACHES TO THE DESIGN OF ALLOSTERIC PROTEINS.