IGEM:UNAM/2009/Notebook/Modeling logbook Claudia/2010/03/12
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Objective: Ask for information about LovTap
I have been in touch with Dr. Devin Strickland the designer of LovTAP, in order to get advices to work with LovTap.
He recommended us the following:
You should take a look at Figure 2.4.2 of my dissertation  and try to figure out why that experiment didn't work. You may understand it already, but my interpretation is that LovTAP was being expressed at too high a level. I'd suggest getting the expression level down as much as possible. Also, whatever your reporter is (LacZ, GFP, etc.), I'd recommend putting a degradation tag on it, as in the classic Elowitz and Leibler "Repressilator" system.
See below the figure 2.4.2 information that Devin cited.