IGEM:UNAM/2009/Notebook/IGEM project/2011/08/04

From OpenWetWare
Jump to: navigation, search
Igem-logo-150px.png iGEM Project name 1 Report.pngMain project page
Resultset previous.pngPrevious entry      

Gel of plasmids

  • Today I did a gel from the extraction from 25/june/2011, to confirm if the extraction was cleaned.

Gel 18. Plasmid

Line 1 -> 3 μl of plasmid J23112A + 1 μl of dye.
Line 2 -> 3 μl of plasmid J23112B + 1 μl of dye.
Line 3 -> 3 μl of plasmid J23112C + 1 μl of dye.
Line 4 -> 3 μl of plasmid J23112D + 1 μl of dye.
Line 5 -> 3 μl of plasmid J23113A + 1 μl of dye.
Line 6 -> 3 μl of plasmid J23113B + 1 μl of dye.
Line 7 -> 3 μl of plasmid J23113C + 1 μl of dye.
Line 8 -> 3 μl of plasmid J23113D + 1 μl of dye.
Line 9 -> 3 μl of plasmid J23114A + 1 μl of dye.
Line 10 -> 3 μl of plasmid J23114B + 1 μl of dye.
Line 11 -> 3 μl of plasmid J23114C + 1 μl of dye.
Line 12 -> 3 μl of plasmid J23114D + 1 μl of dye.
Line 13 -> 3 μl of plasmid J23115A + 1 μl of dye.
Line 14 -> 3 μl of plasmid J23115B + 1 μl of dye.
Line 15 -> 3 μl of plasmid J23115C + 1 μl of dye.

Gel 19. Plasmid

Line 1 -> 3 μl of plasmid J23115D + 1 μl of dye.
Line 2 -> 3 μl of plasmid J23116A + 1 μl of dye.
Line 3 -> 3 μl of plasmid J23116B + 1 μl of dye.
Line 4 -> 3 μl of plasmid J23117A + 1 μl of dye.
Line 5 -> 3 μl of plasmid J23117B + 1 μl of dye.
Line 6 -> 3 μl of plasmid J23117C + 1 μl of dye.
Line 7 -> 3 μl of plasmid J23117D + 1 μl of dye.
Line 8 -> 3 μl of plasmid J23118A + 1 μl of dye.
Line 9 -> 3 μl of plasmid J23118B + 1 μl of dye.
Line 10 -> 3 μl of plasmid J23118C + 1 μl of dye.
Line 11 -> 3 μl of plasmid J23119A + 1 μl of dye.
Line 12 -> 3 μl of plasmid J23119B + 1 μl of dye.
Line 13 -> 3 μl of plasmid J23119C + 1 μl of dye.
Line 14 -> 3 μl of plasmid J23119D + 1 μl of dye.
Line 15 -> Green Ladder.


Results

  • The plasmid was not visible in the gels, so the extraction was not successful.

Gel 18. Plasmid

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15.
J23112A J23112B J23112C J23112D J23113A J23113B J23113C J23113D J23114A J23114B J23114C J23114D J23115A J23115B J23115C
I I I I I I I I I I I I I I I


Gel 19. Plasmid

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15.
J23115D J23116A J23116B J23117A J23117B J23117C J23117D J23118A J23118B J23118C J23119A J23119B J23119C J23119D Ladder
I I I I I AM AM I I I I I I I 2μl

Code:

  • C: clean
  • A: abundant (more concentrated than our ladder strong bands)
  • D: dirty
  • B: barely visible
  • I: Invisible
  • M: isomorfical bands present.