IGEM:UNAM/2009/Notebook/IGEM project/2011/06/08

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Ligation of Blunting

  • 1. Put 24 μl of destilled water to dilute DNA.
  • 2. Put 2 μl of Bluntig DNA.
  • 3. Put 3μl of Buffer T4 lis 10X.
  • 4. Put 1 μl of T4 DNA ligase.
  • 5. Put in the Centrifugate for 20 seconds to 10 mil rpm.
  • 6. Put to 25°C for 10 hours.
We did two tubes of DNA blunting with the last method.