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Ligation of PBBMRCS-5 plasmid with PSB1T3 plasmid and transformation of these plasmids
PSB1T3 plasmid was amplified by PCR, this plasmid has RFP and PBBMRCS-5 plasmid has a gentamicin resistance.
17.5 μl digestion (1) 22. 5 μl digestion (2) 7.5 μl of no-digested plasmid
1. Put 2.5 μl of DNA in 10 μl of:
Wait for 8 hours.
Control (no transformed)(1) Competent cells + ligate plasmid (enzymes) Competent cells + enzymes (without plasmid)