IGEM:UNAM/2009/Notebook/IGEM project/2011/06/01

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Grow Escherichia Coli DH5-α

Abstract

  • Today I did three petri dishes with the bacteria Escherichia Coli DH5-α, which has the PBBMRCS-5 plasmid.
  • Besides I did a agarose gel of the PBBMRCS-5 plasmid.

Grow E. coli DH5-α

  • 1. Prepare LB medium.
  • 2. Put 100 μl of gentamicin.

We do three tubes:

  • 1. Control tube -> LB solid medium + gentamicin.
  • 2. Bacteria tube -> LB solid medium + gentamicin + Escherichia coli DH5-α.
  • 3. Bacteria tube -> LB solid medium + gentamicin + Escherichia coli DH5-α.


Note: The PBBRMCS-5 plasmid give to the bacteria resistance to gentamicin.

Gel of PBBMRCS-5 plasmid

  • Line 1st -> 5 μl of Green ladder.
  • Line 2nd -> 2.5 μl of plasmid + 2.5 μl of dye. (1)
  • Line 3rd -> 2.5 μl of plasmid + 2.5 μl of dye. (2)
  • Line 4th -> 5 μl of Green ladder.

Note: Previously the plasmid PBBMRCS-4 was digested with XBA I and Hind II.