Medium of Chlamydomonas reinhardtii
Abstract
Today Miriam gave me the protocol to make TAP medium.
Tap Medium
| Reagent
|
Quantity
|
| Beijerincks 20X
|
50 ml
|
| Acetic acid
|
1 ml
|
| Tris
|
2.42g
|
| Phosphate solution 1M pH 7
|
1 ml
|
| Trace Element
|
1ml
|
Gauging to 1 liter.
Beijerincks 20X solution
| NH4Cl
|
8 g
|
| CaCl2·H2O
|
1 g
|
| MgSO4· 7H2O
|
2 g
|
Trace Element Solution
| Reagent
|
Quantity
|
| EDTA sodium salt
|
50 g
|
| ZnSO4·7H2O
|
22 g
|
| H3BO3(a)
|
11.4 g
|
| FeSO4·7H2O
|
4.99 g
|
| MnCl2·H2O
|
5.06 g
|
| CoCl·6H2O(b)
|
1.61 g
|
| CuSO4·5H2O
|
1.57 g
|
| (NH4)6Mo7O24·H2O
|
1. 10g
|
(a) Heat to dissolve
(b) Shake to disolve
Dissolve each element independently.
- Boric acid in 200 ml.
- Sulphate if zinc in 200 ml.
- EDTA in 250 ml.
- Other elements in 50 ml.
- 1. Put all solutions in a flask and add the EDTA, solution has a purple color.
- 2. Lead to boiling point, until the solution has a green color.
- 3. Decrease a little the temperature (no less of 70 °C), set pH between 6.5-6.8 with KOH to 20%.
- 4. Gauge to 1 liter.
- 5. Leave the solution in a flask until the solution get a purple color.
- 6. Remove the residue of reddish brown color by filtration.
| 
iGEM Project name 1
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