IGEM:UNAM-Genomics Mexico/2009/Notebook/HRG personal log/2011/05/21
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21/05/2011 06:04 a.m.
I have had little experience in the wet lab. This is the first time I have encountered myself in the laboratory alone.
Before taking the gel out, I had to know know, which was the aim of this gel? (I did not prepare de DNA with which it was loaded and it was not stated in the notebook) I decided to look at the concentration of the gel and based on that decided it was not a gel for extraction.
I took the gel out and placed it on the transilluminator
The results were as follows:
Lane 1 Ladder (1Kb Fermentas) Ladder
Lane 2 Forward Negative
Lane 3 Reverse Negative
Lane 4 No DNA Negative
Lane 5 No oligos Negative
Lane 6 5mg DNA Band
Lane 7 10mg DNA Band
Lane 8 Ladder (1Kb Fermentas) Ladder