IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/09/04

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  • Repeating the standardization


Transformation results

I came to see if some colonies grew up in the yesterday's transformation. I only see colonies in the petri dish corresponding to the reaction of ligation one (see my yesterday logbook). It makes sense because on the gel the PCR was not observable so with this ratio (1:4) insert/host was expected to be more efficent the ligation. However the colonies were not red yet, so I picked up all of them (~30) and I re-platted on new petri dishes with gentamicin LB. Tomorrow I will see if the colonies get red.