IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/08/27
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Abstract
MainStandardizationPCR result The result of the yesterday's PCR are shown in the following electrophoresis gel (120V X 35 min): Lanes: 1. Ladder 500bp. 2. PCR The band corresponds to the correct size (~1.5 kb) and is clean enough to further experiments. PCR and plasmid digestion Before I did the PCR product digestion, I cleaned up with the purification kit by Roche In order to do the ligation by sticky ends I also did the double digestion by apaI and sacI of both, the pBBRMCS-5 plasmid and the PCR of the RFP. The reactions were done with following compounds: RFP PCR digestion
pBBR1MCS-5 digestion
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