IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/08/27
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The result of the yesterday's PCR are shown in the following electrophoresis gel (120V X 35 min):
1. Ladder 500bp. 2. PCR
The band corresponds to the correct size (~1.5 kb) and is clean enough to further experiments.
PCR and plasmid digestion
Before I did the PCR product digestion, I cleaned up with the purification kit by Roche
In order to do the ligation by sticky ends I also did the double digestion by apaI and sacI of both, the pBBRMCS-5 plasmid and the PCR of the RFP.
The reactions were done with following compounds:
RFP PCR digestion