IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/08/22
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Today I did a PCR from the pSB1C3 plasmid amplifying all the backbone, because I will need it to isert our parts.
I did the PCR with the RTTH enzyme, according to the following steps.
1. I prepared the two mixes, which were:
2. Once ready a I put the mix into the thermocylcer, the first cycle was:
3. Then I added the mix 2, after that the following cycle were done:
94°C / 30 seg 60°C / 30 seg 72°C / 3 min
72°C / 5 min 4° C / ∞
I will see the results on wednesday, in an electrophoresis gel.