IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/07/29
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Abstract1. I continued with the experiment of plasmid's stability of pBBR1MCS-5. 2. I continued with the conjugation of the standard pBBR1MCS-5 into R. etli. 3. I ran a Paulina's gel. MethodsPlasmid stability
1. Vortex the 1 ml culture. 2. Take 100μl of the previous culture and put them in 900 μL of liquid medium of antibiotic. This is the first dilution. 3. Vortex 30 times the dilution. 4. Repeat the steps 2 and 3 until reach the desired dilution. I did it until reached the 8th dilution, becuase the original culture was 48 hours old and there were a lot of bacteria. Then I plaqued 100 μL of the dilutions 5, 6, 7 and 8.
1. Vortex the 1 ml culture. 2. Take 100μl of the previous culture and put them in 900 μL of liquid medium of antibiotic. This is the first dilution. 3. Vortex 30 times the dilution. 4. Repeat the steps 2 and 3 until reach the desired dilution. I did it until reached the 9th dilution, becuase the original culture was 48 hours old and there were a lot of bacteria. Then I plaqued 100 μL of the dilutions 6, 7, 8 and 9.
ConjugationPauilna's gelThe following is a gel (120V x 35 min) which Paulina asked me to do it. Lanes: 1. Isolation 15 2. Isolation 2 3. Isolation 3 4. Isolation 4 5. Isolation 5 6. Isolation 5 7. Isolation 7 8. Isolation 1 9. Isolation 8 10. Isolation 9 11. Isolation 10 12. Isolation 11 13. Isolation 12 14. Isolation 13 15. Isolation 14
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